The rapidSTRIPE Listeria Assay is a fast, highly specific tool for detecting the genus Listeria monocytogenes in food after standard culturing. This involves a subsequent, specific amplification reaction in the same reaction vessel, combined with a hybridization step. In the final step, the PCR products are applied to a user-friendly test strip; the appearance of a test line indicates positive results. A second control line (in the form of a conjugate control) confirms that that lateral flow strip (LFS) is working properly. The kit is ready-to-use and contains all of the reagents and consumables needed for the PCR, hybridization and final detection.