A number of rapidSTRIPE Assays has been optimized for detecting Tick pathogens.
Once the nucleic acid has been extracted from ticks (using the blackPREP Tick DNA Kit or blackPREP Tick DNA/RNA Extraction Kit), the DNA is introduced into a specific combination reaction consisting of amplification and subsequent hybridization. Both of these reactions occur uninterrupted within the same reaction vessel. The final step involves loading the hybridized amplification product onto a Lateral Flow Strip. A positive test result for pathogen is confirmed by the appearance of a test line. The strip also includes a control line (conjugate control) to ensure the reaction is working properly. The Lateral Flow Strips developed for this assay are stable for long periods and can be archived.