QX200™ Droplet Digital™ PCR System (186-4001)

Manufacturer Bio-Rad  |   Model: 186-4001  |  Available Worldwide
4.4
/
5.0
  |  8 reviews



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Average Rating: 4.4
8 Scientists have reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money


Bio-rad ddPCR highly effective.
Rating: 5.0

  • Application Area: Sensitive detection of viral rna

"Effective in detecting viral mutations. High quality results with easy use. Highly recommended. Great company with extensive support. Cost effective."

Review date: 30 Mar 2016 | QX200™ Droplet Digital™ PCR System (186-4001)
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Cleveland Clinic Foundation



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Simple but efficient system
Rating: 4.3

  • Application Area:Gene quant

"It provides an absolute count of target DNA copies per input sample without the need for standard curves which makes it easier to use compared to real-time PCR in which there may be variations due to reference gene amplification. Also simple and easy-to-use workflow with 96-sample throughput does not require multiple dilution steps. "

Review date: 24 Mar 2016 | QX200™ Droplet Digital™ PCR System (186-4001)
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Horizon Discovery



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
A must in every lab.
Rating: 4.7

  • Application Area:genotyping

"Very easy to operate, providing excellent results. Very reliable as well!"

Review date: 24 Mar 2016 | QX200™ Droplet Digital™ PCR System (186-4001)
  • Status:

    Reviewer

  • Member since: 2014

  • Organization: King AbdulAziz University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0

  • Application Area:MDx

"When your qPCR detecting a Cq over 32, the best technique is to use the highly sensitive QX 200 ddPCR."

Review date: 06 Oct 2015 | QX200™ Droplet Digital™ PCR System (186-4001)
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Lund University



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.0

  • Application Area:Plasma

"Very useful product specially CNV and rare mutation analysis"

Review date: 21 Sep 2015 | QX200™ Droplet Digital™ PCR System (186-4001)


  • Status:

    Reviewer

  • Member since: 2015

  • Organization: AltraTech Ltd



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Rating: 4.7

  • Application Area:PCR

"I was very happy with the results I achieved with the Bio-Rad QX200™ Droplet Digital™ PCR System and found it easy-to-use."

Review date: 25 Mar 2015 | QX200™ Droplet Digital™ PCR System (186-4001)
  • Status:

    Reviewer

  • Member since: 2014

  • Organization: University of Michigan



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0

  • Application Area:We use it for two purposes: Genotyping (copy number) and gene expression

"So far, the droplet technology has been pretty straightforward to use and teach to others. It has thus far replicated several differential gene expression and copy number studies in our lab."

Review date: 17 Nov 2014 | QX200™ Droplet Digital™ PCR System (186-4001)
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: Autonomous University of Tamaulipas



  • Ease of use
    4 out of 5
    After sales service
    2 out of 5
    Value for money
    2 out of 5
Rating: 2.7

  • Application Area:Polymorphism

"It worked well but it needs a better support life. Without it, it doesn't work well enough. I would recommend to other scientists."

Review date: 29 Nov 2013 | QX200™ Droplet Digital™ PCR System (186-4001)

Use the QX200 Droplet Digital PCR (ddPCR™) system, the most precise and sensitive PCR solution, for absolute quantification of target DNA molecules.

Droplet Digital PCR (ddPCR™) is Bio-Rad's unique digital PCR technology. With unrivaled precision, ddPCR provides absolute quantification of target DNA or RNA molecules without the use of standard curves. ddPCR addresses the lack of scalable and practical technologies for digital PCR implementation. The new QX200 ddPCR system puts this powerful technology in your hands, ready to unveil new worlds of research at previously unattainable levels.

Droplet Digital™ PCR system, includes droplet generator, droplet reader, laptop computer, software, associated component consumables, for EvaGreen or probe-based digital PCR applications.

The QX200 droplet generator is used to partition ddPCR reaction mix into 20,000 nanoliter-sized droplets. After PCR on a thermal cycler, droplets from each sample are analyzed individually on the QX200 droplet reader. Droplets are sipped and the singulator unpacks the emulsified droplets and streams them in single file past a two-color optical detection system in a serial manner. Up to 96 samples can be processed per run. The PCR-positive and PCR-negative droplets are counted to provide absolute quantification of target DNA in digital form. Alternatively, amplified products can be extracted from droplets following PCR for downstream applications, such as sequencing or cloning.

Featured at Bio-Rad AACR 2014. Winner of the SelectScience Scientists' Choice Awards Best New Life Sciences Product of 2013.


Features:

  • Flexible Digital PCR Chemistry – optimized for TaqMan hydrolysis probe and EvaGreen applications.
  • Reduced Bias from Amplification Efficiency and PCR Inhibitors.
  • Flexible Assay Setup – scalable for high sensitivity or high throughput.
  • Convenient Assay Design – standard curves are not required.
  • Simple workflow with 96-sample throughput.

Applications:

  • Cancer biomarker studies and copy number variation
  • Pathogen detection
  • Next generation sequencing
  • Gene expression analysis
  • Environmental monitoring
  • Food testing

Product Overview

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QX200™ Droplet Digital™ PCR System (186-4001)

Manufacturer Bio-Rad  |  Available Worldwide

4.4 / 5.0 | 8 reviews