QuikChange XL Site-Directed Mutagenesis Kit, 30 Rxn by Agilent Technologies

The original QuikChange Site-Directed Mutagenesis Kits eliminate the need for subcloning into M13-based bacteriophage vectors and for ss-DNA rescue. This makes site-directed mutagenesis studies simple and reliable since it allows oligo-mediated introduction of site-specific mutations into virtually any double-stranded plasmid DNA. The XL version of the kit is specialized for efficient mutagenesis of large (4 -14 kb) or otherwise difficult-to mutagenize plasmid templates and features components specifically designed for more efficient DNA replication and bacterial transformation. The QuikChange Multi system allows mutagenesis at multiple sites (up to 5) in a single round, using a single oligonucleotide per site.

• >80% mutagenesis efficiency for 1 site; >55% mutagenesis efficiency for 3 mutations simultaneously
• Efficient and accurate linear amplification minimizes unwanted errors
• Free access to Primer Design Program

For Research Use Only. Not for Use in Diagnostic Procedures.