The Quick Western Kit – IRDye® 680RD provides a universal detection reagent that can be combined with the primary antibody incubation step, eliminating the need for a secondary antibody incubation step. This reduces the overall time to complete a Western blot and provides the advantages of near infrared detection.
The kit can be used to detect primary antibodies from a variety of hosts and has been shown to recognize primary antibodies to recombinant tagged proteins (i.e. 6X His, Myc, DDK, etc.) IRDye 680RD Detection Reagent is known to have high specificity for IgG from human, mouse, rabbit, guinea pig, goat, sheep, pig, cow, cat, dog and donkey. The Detection Reagent is known to have lower specificity for rat, horse, and hamster. The Detection Reagent will not detect primary antibodies from a chicken host. The Detection Reagent has been specifically tested and qualified for Western blot applications. If additional specificity and/or affinity are required, please use IRDye conjugated secondary antibodies for detection.