QUANTIFOIL® – Holey Carbon Films
| Hamed Heidari Mezerji|
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Member since 2012
EMAT UNIVERSITY OF ANTWERP
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"My work with TEM and STEM tomography requires the preparation of ultra clean TEM samples. Quantifoil is one of the best options we have come up with. We are using this regularly in daily experiments. Of course, I would recommend it to a colleague. We have not experienced the after sales service so it has a neutral score of 3/5."
QUANTIFOIL® is a perforated support foil with pre-defined hole size, shape and arrangement. It has advantages in electron microscopy (EM) or low-energy electron point source (LEEPS) microscopy when compared with conventional holey film.
QUANTIFOIL® is offered with circular and square, orthogonal arranged holes. Films with different hole sizes and bar widths are available. Carbon is the standard material that makes the foil.
QUANTIFOIL® is a superior quality of holey carbon film, which facilitates the use of automation in TEM. (e.g. The National Resource for Automated Molecular Microscopy, at the Scripps Research Institute, has developed a system, called Leginon, for automatically acquiring images from a transmission electron microscope).
QUANTIFOIL R 2/2 QUANTIFOIL ® with circular holes is used in cryo-electron tomographic reconstruction. The roundness of the holes is advantageous with respect to the formation of an ice layer of constant thickness. The whole size chosen depends on the magnification used, and on whether or not one wishes to include support film in the image. Assessment of the image quality is easier when foil is included in the picture, because the power spectrum of a foil is stronger than that of unsupported ice.
The surface properties of QUANTIFOIL® holey carbon support film, especially the wetting properties, may have to be adapted according to one’s particular requirements. Untreated aging QUANTIFOIL® tends to be hydrophobic. Hydrophillicity of the foil can be achieved by glow discharging in residual air or by metal coating.
QUANTIFOIL® in low-energy electron point source (LEEPS) microscopy. Quantifoil® with a regular pattern is required in order to be able to distinguish an object, which is spanned over a hole. An object cannot be discriminated from the support in the case of conventional holey support film. (H.W. Fink & C. Schonenberger, University of Basel, used QUANTIFOIL® for the measurement of electrical current through DNA molecules.)
The foil is ~12 nm thick and mounted on either copper, nickel or gold grids with either square or round holes of different sizes.
Holey films with 2µ round holes are used at magnifications between 30,000x and 40,000x.