Status:
ReviewerMember since: 2022
Organization: CSIR-IMTECH
Good yield and easy to use
Application Area: To isolate Plasmid DNA from DH5alpha cells to transfect in mammalian cells
"Obtained a great yield, best in the market when compared to other kits that i have usedand also easy to use, but its somewhat on expensive side, if price can be lowered it will be a great help"
Status:
ReviewerMember since: 2021
Organization: San Diego Biomedical Research Institute
Must use.
Application Area:Measuring replication timing in different cell lines.
"Quick, easy and great yield. Best kit for plasmid prep."
Status:
ReviewerMember since: 2020
Organization: University of Arkansas for Medical Sciences
Dependable workhorse for plasmid purification.
Application Area:Small scale plasmid purification
"Kit represents the gold standard for plasmid preparation. While a little pricey, the Qiagen miniprep kit returns quality DNA for Sanger sequencing. Only complaint is that there is no lid for the columns, which leads to more buffer coating the inside of the centrifuge."
Status:
Expert ReviewerMember since: 2016
Organization: University of North Carolina at Charlotte
Great results for molecular biology experiments.
Application Area:Easily extract plasmid from various samples
"Very effective plasmid extraction kit with easy steps to follow. Straightforward to use, you simply need to follow the instructions and get the higher amount and good quality of plasmid DNA which can be further use to sequencing. Great product and can be efficiently use for plasmid extraction from a range of samples."
Status:
ReviewerMember since: 2018
Organization: Institut für Biochemie und Molekularbiologie (Universitätsklinikum Bonn)
A great kit for plasmid purification for a very reasonable price.
Application Area:Recombinant DNA plasmid isolation
"The kit is easy to use. Rapid and effective, I was able to generate a great amount of different mutants for my gene of study in a few weeks and I got to enough plasmid DNA for further experiments (transfection of insect cells for recombinant protein expression). For each construct I could purify DNA with high yield and high purity. A great kit for plasmid purification for a very reasonable price."
Status:
ReviewerMember since: 2006
Organization: Johns Hopkins University
Great produt, easy to use, works every time.
Application Area:Cloning and expressing enzymes
"I hadn’t done a plasmid “miniprep" since 2008 until today, when I prepped four. What used to be a real chore, is now as easy as rolling off a log with the use of the QIAGEN Miniprep Kit. Science marches on!"
Status:
ReviewerMember since: 2018
Organization: D Y Patil University
Great results, great purity.
Application Area:Plasmid extraction of algae and chlamydonas cultures
"It a very easy to use the mini-prep kit. If the instructions are followed clearly and thoroughly, a high level of purity of plasmids can be achieved. I have extracted over 15 samples using this kit."
Status:
Expert ReviewerMember since: 2015
Organization: Columbia University
Time -saving and easy to use for plasmid DNA
Application Area:purification of plasmid DNA
"Consistently good yield, reliable and easy to use kit for plasmid DNA purification"
Status:
ReviewerMember since: 2018
Organization: Horizon Discovery
Highly recommend this kit for plasmid minipreps
Application Area:DNA extraction from bacteria
"Very easy to use kit with clear and straight forward instructions. I have consistently managed to get excellent DNA yield by using this kit."
Status:
Advanced ReviewerMember since: 2015
Organization: University college London
I seriously doubt nobody can offer a better kit for the same price. Best kit ever
Application Area:Extract plasmid DNA from bacterial cultures
"Highest quality yield, super easy protocol, user friendly instructions and great value for money."
Status:
ReviewerMember since: 2013
Organization: Louisiana State University
Application Area:Cloning
"I've used this kit, and I am completely satisfied with its ease of use and the purity of the plasmid that was suitable for cloning."
Status:
ReviewerMember since: 2012
Organization: Mclab
Application Area:DNA Preparation
"The one problem is that if the user follows the instruction to elute DNA from the column, the DNA is not good for sequencing because it has too much ethanol. The correct step is to spin dry the column for over 10 min at full speed before elution."
QIAGEN Plasmid Kits provide gravity-flow, anion-exchange tips for purification of transfection-grade plasmid DNA. Lysate clearing and isopropanol precipitation are achieved by centrifugation.