The QBT™ Fatty Acid Uptake Assay is a single-step, homogeneous, fluorescent assay for monitoring real-time uptake kinetics of fatty acid transport proteins.
The kit employs a BODIPY® -dodecanoic acid fluorescent fatty acid analog that becomes activated by acyl-CoA attachment; is incorporated into di-and triglycerides; and accumulates in intracellular lipid droplets. In addition, the BODIPY analog is a known substrate for fatty acid transporters since its uptake by adipocytes can be competed by non-labeled fatty acids. The elimination of radioactive compounds results in easier reagent handling, reduced disposal costs and eliminates safety risks associated with radiolabel assays.
Assay kit protocols are pre-optimized and validated for use on SpectraMax® Fluorescent and Multimode Microplate Readers and are available in Explorer (5 assay plates, 1 plate/vial), Bulk (50 assay plates, 10 plates/vial) configurations.
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