Q5® Site-Directed Mutagenesis Kit by New England Biolabs Inc.

The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase along with custom mutagenic primers to create insertions, deletions and substitutions in a wide variety of plasmids. After PCR, the amplified material is added directly to a unique Kinase-Ligase-DpnI (KLD) enzyme mix for rapid, room temperature circularization and template removal. Transformation into high-efficiency NEB 5-alpha Competent E. coli, provided with the kit, ensures robust results with plasmids up to at least 14 kb in length.

Q5® Site-Directed Mutagenesis Kit Features:

• Non-overlapping primer design ensures robust, exponential amplification, generating a high percentage of desired mutations from a wide range of templates
• Intramolecular ligation and transformation into NEB high-efficiency competent cells results in high colony yield
• Extremely low error rate of Q5 Hot Start High-Fidelity DNA Polymerase reduces screening time
• Hot start polymerase enables room temperature reaction set-up
• DpnI background reduction permits a wide range of starting template concentrations
• Use of standard primers eliminates additional expenses from phosphorylated or purified oligos
• Easy-to-use PCR master mix and unique multi-enzyme KLD mix offer convenience and quality
• Rapid and direct treatment step proceeds at room temperature in 5 minutes

Q5® Site-Directed Mutagenesis Kit Applications:

• Difficult PCR
• Fast PCR
• High-Throughput PCR
• High-Yield PCR
• Long Range PCR
• Multiplex PCR
• Routine PCR
• Site Directed Mutagenesis