Status:
ReviewerMember since: 2023
Organization: UC Davsi
Excellent polymerase, my go-to for everything PCR related
Application Area: PCR
"Works extremely well and is super reliable. I love this polymerase!"
Status:
Advanced ReviewerMember since: 2019
Organization: Leibniz Zentrum für Marine Tropenforschung
Super amplification without further PCR optimazation steps.
Application Area:PCR with difficult DNA templates
"We have finally found a perfect polymerase that works with the first PCR and does not need to be further optimized. We work a lot with plant DNA in the field of mangroves, macroalgae, seaweed. These templates in particular have many inhibitors (polyphenols and polysaccharides) and often make amplification difficult. NEB has a comparatively inexpensive alternative to the conventional, expensive polymerases."
Status:
Expert ReviewerMember since: 2020
Organization: Montana State University
Great and reliable results.
Application Area:Molecular cloning
"High quality product with flexibility in annealing temperature during PCR. Also can be relied on PCR products with no errors."
Status:
Expert ReviewerMember since: 2020
Organization: Montana State University
Great product has made my experiment go smooth with high quality results.
Application Area:PCR for gene cloning
"High flexibility with melting temperature, and good proof reading ability. "
Status:
ReviewerMember since: 2021
Organization: Miami University
Great product!
Application Area:DNA cloning
"I always achieve high quality and reproducible results using this product."
Status:
Advanced ReviewerMember since: 2019
Organization: Unviersity of Chicago
Efficient PCR amplification for difficult templates.
Application Area:PCR for recombinant DNA synthesis
"The formulation that we purchased is the High Fidelity Hot Start Master Mix. Remarkably simple to use given the formulation without the need to pipette individual components. Accounting for the slightly higher annealing temperature needed for Q5, we were able to obtain several PCR products that were not amplified with our other favorite proofreading polymerase. It seemed to work particularly well with some of our genomic templates. Overall a great product."
Status:
ReviewerMember since: 2017
Organization: University Hospital Southampton NHS Trust
Great Reagent
Application Area:NGS amplicon library preparation
"Great easy to use reliable Master mix. A proof reader with 3-5' exonuclease activity, great amplification of many targets but can permit binding and amplification to pseudogenes so careful with your designs."
With an error rate > 100-fold lower than that of Taq DNA Polymerase and 12-fold lower than that of Pyrococcus furiosus (Pfu) DNA Polymerase, Q5 High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons.
Q5 High-Fidelity DNA Polymerase is supplied with an optimized buffer system that allows robust amplification regardless of GC content. The 5X Q5 Reaction Buffer contains 2 mM MgCl2 at final (1X) reaction concentrations and is recommended for most routine applications.
For GC-rich targets (≥ 65% GC), amplification can be improved by the addition of the 5X Q5 High GC Enhancer. Q5 High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes.
Q5® High-Fidelity DNA Polymerase Applications: