Expanded bed adsorption (EBA) chromatography is a technology ideally suited for rapid processing of large volumes of crude biological feedstocks and for the purification of plasmids, IgG and various recombinant proteins. Rapid, direct capture of target proteins by expanded bed adsorption offers considerable operational benefits:
• Significantly reduces process cycle time by eliminating post-fermentation clarification
• Improves product quality and yield
• Reduces total capture-step costs by 20 - 40%
Existing ion exchange sorbents for EBA have not been designed for optimal productivity in industrial operations. Protein binding typically requires significant feedstock dilution, unacceptably increasing process volumes and load times. In addition, these sorbents are incompatible with high biomass loads (>10% ww/v); exceeding these levels can only be achieved by a reduction in operating flow rates, substantially increasing process cycle duration.
BioSepra® Q and CM HyperZ™ are members of a new family of high-density sorbents from Pall®, specifically designed for high productivity expanded bed chromato¬graphy and efficient capture of biomolecules directly from crude, unclarified feedstocks in a single pass operation.
Higher biomass and flow rate without sacrificing capacity.
The HyperZ product range has been designed to deliver the broadest useful linear flow range possible of any commercial expanded bed sorbents. Due to a high charge density in its hydrogel moiety, the CM HyperZ sorbent has unique protein binding properties in the presence of salt. This provides significant benefits at process scale, like avoiding or limiting the dilution of large volumes of feedstream.
Manufacturer Pall Life Sciences Products - Biopharmaceutical Division
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