Q, S, DEAE, CM Ceramic HyperD® Ion Exchange Sorbents by Pall Life Sciences Products - Biopharmaceutical Division

High Productivity from Rigid, Composite Sorbents
Q, S, DEAE, CM Ceramic HyperD ion exchangers are high capacity sorbents specially designed for the efficient and scaleable purification of biomolecules from a variety of feedstocks. Of particular value in process-scale application, these sorbents are designed to maintain high dynamic binding capacity (DBC) under conditions where conventional sorbents display significant capacity or productivity limitations.

The "Gel in a Shell" Design Provides Rapid Mass Transfer and Effective Binding
BioSepra Ceramic HyperD ion exchangers employ a high-capacity hydrogel polymerized within the gigapores of a rigid ceramic bead. This design combines the desirable characteristics of a soft, high-capacity hydrogel with the absolute dimensional stability of a rigid ceramic bead. Ceramic HyperD sorbents do not shrink or swell with changes in pH or conductivity. Abundant ion exchange sites in the hydrogel are highly accessible to protein molecules. Proteins diffuse rapidly within the hydrogel, facilitating rapid uptake of product. This mechanism of mass-transfer – known as enhanced diffusion – allows the sorbent to operate free of operational constraints typically encountered with conventional macroporous ion exchange sorbents.

Capture Product at High Linear Velocity
Feedstock can be processed rapidly and efficiently. Dynamic binding capacity with Ceramic HyperD ion exchangers is virtually independent of linear velocity at values typically used – or sought – in laboratory or process scale separations. The pronounced flow-dependent behavior associated with traditional ion exchangers is not observed.

Efficient Harvest from Dilute Feedstock
Using Ceramic HyperD ion exchange sorbents, higher dynamic binding capacity is obtained with dilute feedstock than with concentrated feedstock. This behavior – contrary to that observed with traditional sorbents – illustrates that these novel materials operate by a mechanism that is fundamentally distinct from that associated with classical macroporous sorbents. In many applications, product can be harvested from dilute feedstock without need for preliminary concentration, thereby enhancing the productivity of the overall purification scheme.

Recover Product from Feedstock of Moderate Ionic Strength
The extraordinarily high ligand density present within the hydrogel of the CM Ceramic HyperD F ion exchange sorbent allows for effective binding even when product is present in feedstock of moderate ionic strength. For example, monoclonal antibody has been harvested from feedstock of 19 mS conductivity – equivalent to 180 mM salt. Thus, in many applications feedstock can be applied without preliminary diafiltration or dilution.

Absolute Dimensional Stability, Excellent Pressure/flow Characteristics, Easy to Pack
Ceramic HyperD ion exchangers form very stable beds. They do not swell or shrink in response to changes in pH, ionic strength or flow rate. Indeed, the sorbents are stable at pressures up to 70 bar. Since the bead does not deform under fluid flow, the sorbent provides linear pressure/flow behavior, and has characteristics well suited to operation using low-pressure columns and equipment. For example, in a pilot-scale column (9 cm ID x 16 cm) operated at 500 cm/h, operating pressure generated by the sorbent is less than 2 bar. Equally important, column packing is accomplished quickly and easily owing to the dense nature of the Ceramic HyperD beads.

Robust, Safe and Documented for Validation
Ceramic HyperD sorbents are manufactured in BioSepra S.A. ISO 9001 registered manufacturing facility. Extensive experience on batch-to-batch reproducibility (see Table 3) guarantees the reliable performance and supply you need for your process. The sorbents can be treated with NaOH for efficient cleaning and sanitization. Ceramic HyperD sorbents are used in a number of registered products, as well as in many clinical and preclinical trials, in columns larger than 500 liters.