Status:
Expert ReviewerMember since: 2016
Organization: University of North Carolina at Charlotte
Excellent quality and great outputs
Application Area: To reduce the viscosity from the samples before PCR run
"This is a new machine that we bought in our lab recently. I have learned to use this machine for the sealing the PCR plate and it actually works. Provides the better results as compare to the PCR plates run when you do the manual sealing. It reduces the viscosity and improves the amplification and error between the replicates. Happy to have it at my lab and will recommend to have in yours especially, if you are working in the field of qPCRs."
Status:
Advanced ReviewerMember since: 2014
Organization: Northwestern University
Application Area:Sealing 384 plates with clear and aluminum heat seals
"The sealer was very easy to set up and ready to seal pretty much out of the box. Works well, eliminates user error inherent in manual application of seals that leads to evaporation at corner and edge wells. My only complaint is the price of the seals, I would expect them to be cheaper than adhesive seals since we have to buy the sealer, and unfortunately they are not."
Use the PX1 PCR Plate Sealer for consistent, uniform sealing across the entire plate, minimizing evaporation during cycling. The small-footprint heat sealer is compatible with a wide range of PCR plates and different types of films and foils.
The intuitive touch-screen interface of the PX1 PCR Plate Sealer shows instrument status, platen temperature, protocol name, set temperature, and time. Sealing protocols can be stored and easily edited. The sealer can be programmed to automatically shut down after a user-defined period of idle time. Touching the display after shutdown will reactivate the display and the sealer will begin heating to the last set temperature.
The semi-automated PX1 PCR Plate Sealer provides fast, consistent sealing, removing human variability and increasing reproducibility. Uniform sealing over the whole plate is particularly important for low-volume samples where small amounts of evaporation can have significant effect. In qPCR experiments, differential evaporation from wells can lead to large standard deviations in the number of quantification cycles (Cq).