PureYield™ Plasmid Midiprep System

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PureYield™ Plasmid Midiprep System
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The PureYield™ Plasmid Midiprep System is designed to isolate transfection-quality plasmid DNA. The system provides a rapid method for purification of 100–200μg of plasmid DNA from 50ml bacteria culture. Plasmid DNA can be purified in as little as 30 minutes with the vacuum protocol, greatly reducing the time spent on purification compared to silica resin or other membrane-column methods. An alternative protocol allows purification of over 400μg of high-copy-number plasmid from 250ml of E. coli culture.The PureYield™ Plasmid Midiprep System incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants improves the robustness of sensitive applications such as eukaryotic tran...Read more

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Reviews

8 Scientists have reviewed this product


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Average Rating: 4.2

Ease of use
4 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
Rating: 3.3
Ease of use
2 out of 5
After sales service
5 out of 5
Value for money
3 out of 5
  • Review date: 02 Dec 2013

  • Application Area: In Situ Hybridization

  • PureYield™ Plasmid Midiprep System

"You do need specific equipment, such as particular centrifuge sizes or the vacuum manifold. If you have that it works well but trying to get around that results in lower yields."

Rating: 4.0
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
  • Review date:17 Dec 2012

  • PureYield™ Plasmid Midiprep System

"This worked well for me, I got very reasonable yields and it wasn't difficult to use. I don't have a good benchmark to compare to the Qiagen kit though."
Rating: 4.0
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
  • Review date:16 Dec 2012

  • Application Area:DNA isolation

  • PureYield™ Plasmid Midiprep System

"Instructions are very easy to practice. Even not so skilled undergraduates can do DNA isolations. Midi-prep kits give us a good yield almost all the time."
Rating: 4.0
Ease of use
5 out of 5
After sales service
3 out of 5
Value for money
4 out of 5
  • Review date:16 Dec 2012

  • Application Area:cloning/subcloning/mutagenesis

  • PureYield™ Plasmid Midiprep System

"The kit is definitely quick and easy to use - the 2mL culture size is handy. The disadvantage of PureYield compared to Qiagen kits is the yield, 7.5ug of plasmid max versus 30ug for Qiagen."

Description

The PureYield™ Plasmid Midiprep System is designed to isolate transfection-quality plasmid DNA.

The system provides a rapid method for purification of 100–200μg of plasmid DNA from 50ml bacteria culture. Plasmid DNA can be purified in as little as 30 minutes with the vacuum protocol, greatly reducing the time spent on purification compared to silica resin or other membrane-column methods. An alternative protocol allows purification of over 400μg of high-copy-number plasmid from 250ml of E. coli culture.

The PureYield™ Plasmid Midiprep System incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants improves the robustness of sensitive applications such as eukaryotic transfection, in vitro transcription and coupled in vitro transcription/translation (e.g., TnT® Quick Coupled Transcription/Translation System).

Purification is achieved without isopropanol precipitation of purified plasmid DNA or extensive centrifugation, providing rapid purification from a single method. The system has been designed for use with centrifugation or vacuum (e.g., the Vac-Man® Laboratory Vacuum Manifold).


PureYield™ Plasmid Midiprep System Features & Benefits:

  • Improved Productivity - Vacuum protocol allows plasmid DNA purification in as little as 30 minutes.
  • Confidence in Results - High purity and concentration of plasmid DNA gives proven performance in transfection, in vitro expression and other molecular biology applications.
  • Ease of Use - Simple protocol eliminates tedious high-speed centrifugation, gravity-drip columns, and post-elution alcohol precipitation.
  • Flexibility - PureYield™ membrane column allows purification of large amounts of plasmid DNA, exceeding the capabilities of other midiprep systems.

Product Overview

Product Image

PureYield™ Plasmid Midiprep System

by Promega Corp.

4.2/5.0 (8 reviews)
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money
4 out of 5






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