PureYield™ Plasmid Midiprep System

PureYield™ Plasmid Midiprep System
by Promega Corp.
 4.2/5.0 (8 reviews)

The PureYield™ Plasmid Midiprep System is designed to isolate transfection-quality plasmid DNA. The system provides a rapid method for purification of 100–200μg of plasmid DNA from 50ml bacteria culture. Plasmid DNA can be purified in as little as 30 minutes with the vacuum protocol, greatly reducing the time spent on purification compared to silica resin or other membrane-column methods. An ...read more

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Reviews

AVERAGE RATING: 4.2

8 scientists have reviewed this product

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  • 4 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY

Laura Hinds


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Member since 2013

Organization:
University Of Colorado Boulder
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RATING: 3.3

  • 2 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 3 out of 5VALUE FOR MONEY




Review date: 02 Dec 2013

Application Area: In Situ Hybridization
PureYield™ Plasmid Midiprep System

"You do need specific equipment, such as particular centrifuge sizes or the vacuum manifold. If you have that it works well but trying to get around that results in lower yields."

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Jenna Caldwell


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Member since 2012

Organization:
Stanford University
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RATING: 4.0

  • 4 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 17 Dec 2012

PureYield™ Plasmid Midiprep System

"This worked well for me, I got very reasonable yields and it wasn't difficult to use. I don't have a good benchmark to compare to the Qiagen kit though."

Jayanthi Sanjeevi


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Member since 2010

Organization:
Miami University
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RATING: 4.0

  • 4 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 16 Dec 2012

Application Area: DNA isolation
PureYield™ Plasmid Midiprep System

"Instructions are very easy to practice. Even not so skilled undergraduates can do DNA isolations. Midi-prep kits give us a good yield almost all the time."

Faye Martin


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Member since 2012

Organization:
Colorado State University
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RATING: 4.0

  • 5 out of 5EASE OF USE
  • 3 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 16 Dec 2012

Application Area: cloning/subcloning/mutagenesis
PureYield™ Plasmid Midiprep System

"The kit is definitely quick and easy to use - the 2mL culture size is handy. The disadvantage of PureYield compared to Qiagen kits is the yield, 7.5ug of plasmid max versus 30ug for Qiagen."

SHOUGUANG JIN


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Member since 2011

Organization:
UNIVERSITY OF FLORIDA
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RATING: 4.3

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 3 out of 5VALUE FOR MONEY




Review date: 06 Jul 2011

PureYield™ Plasmid Midiprep System

"Excellent. High quality DNA and easy to use."

Adriana Galvis


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Member since 2010

Organization:
Florida Internatl Univ
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 05 Jan 2011

PureYield™ Plasmid Midiprep System

"Great kit, easy to use with excellent DNA yields."

Sushmita Mustafi


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Member since 2010

Organization:
Florida International University
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 05 Jan 2011

PureYield™ Plasmid Midiprep System

"Quick"

SHIE-CHAU LIU


Status:


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Member since 2008

Organization:
STANFORD UNIVERSITY
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RATING: 3.6

  • 4 out of 5EASE OF USE
  • 3 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 08 Aug 2008

PureYield™ Plasmid Midiprep System

"Easy to use and produces a good yield."

Description

 

The PureYield™ Plasmid Midiprep System is designed to isolate transfection-quality plasmid DNA.

The system provides a rapid method for purification of 100–200μg of plasmid DNA from 50ml bacteria culture. Plasmid DNA can be purified in as little as 30 minutes with the vacuum protocol, greatly reducing the time spent on purification compared to silica resin or other membrane-column methods. An alternative protocol allows purification of over 400μg of high-copy-number plasmid from 250ml of E. coli culture.

The PureYield™ Plasmid Midiprep System incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants improves the robustness of sensitive applications such as eukaryotic transfection, in vitro transcription and coupled in vitro transcription/translation (e.g., TnT® Quick Coupled Transcription/Translation System).

Purification is achieved without isopropanol precipitation of purified plasmid DNA or extensive centrifugation, providing rapid purification from a single method. The system has been designed for use with centrifugation or vacuum (e.g., the Vac-Man® Laboratory Vacuum Manifold).


PureYield™ Plasmid Midiprep System Features & Benefits:

  • Improved Productivity - Vacuum protocol allows plasmid DNA purification in as little as 30 minutes.
  • Confidence in Results - High purity and concentration of plasmid DNA gives proven performance in transfection, in vitro expression and other molecular biology applications.
  • Ease of Use - Simple protocol eliminates tedious high-speed centrifugation, gravity-drip columns, and post-elution alcohol precipitation.
  • Flexibility - PureYield™ membrane column allows purification of large amounts of plasmid DNA, exceeding the capabilities of other midiprep systems.

Product Overview

PureYield™ Plasmid Midiprep System by Promega Corp.
PureYield™ Plasmid Midiprep System




  • 4out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY





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