A Fast Versatile HPLC Column
Hamilton PRP-X300 columns rapidly and easily separate closely related alcohols and organic acids without column heating. The sulfonated poly(styrene-divinylbenzene) support separates samples by a mixed mode mechanism.
The three separation modes are:
- Hydrogen Bonding
- The attraction and retention of sample compounds by the negatively charged sulfonate group.
- Reversed Phase The interaction and retention of the sample compounds by the nonpolar polymeric support.
- Ion Exclusion The process in which ionized samples are excluded from the pores of the support and elute first, while the nonionic compounds elute later. The dominant separation mechanism is determined by sample composition and separation conditions.