Pure nitrocellulose S&S Protran membranes are manufactured using 100% pure nitrocellulose to ensure the highest binding capacity possible. Other membranes referred to as "e;nitrocellulose"e; may actually contain large amounts of cellulose acetate which will lower the protein binding capacity.
High binding, low background In addition to high binding capacity, Protran nitrocellulose membranes inherently have very low background. The superior surface properties of the membrane guarantee superior signal-to-noise ratios, without the need for stringent washing conditions
High retention of small proteins The 0.2 µm pore size of the Protran BA83 nitrocellulose membrane ensures high retention of small samples below 20 kD by reducing "e;blow through"e;. Our 0.45 µm pore size membrane (BA85) is ideal for larger molecular weight samples. BA79 Protran membranes with a pore size of 0.1 µm are for smaller molecules below 7kD.
No methanol pre-wetting step Unlike PVDF membranes, Protran nitrocellulose requires no methanol pre-wetting step. This makes it the membrane of choice for proteins which prefer aqueous environments. Prior to transfer, the membrane is simply wet in water, and then in the transfer buffer. No other pre-treatment steps are necessary.
Stable Protran membranes have the best handling strength of all pure nitrocellulose membranes.
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