The unique PROTEINEER concept includes
The PROTEINEER strategy: Protein Identification
Frequently, Proteomics projects include an initial screening step which aims for the identification of large numbers of proteins. Here, sample throughput and success rate (i.e. the number of positively identified proteins) are key issues.
This task is typically performed via automated MALDI-TOF MS peptide mapping.
Additional MS/MS fragment analyses for further identification and verification can be performed
The PROTEINEER strategy: Protein Characterization
In the later course of Proteomics projects, relevant candidates need to be characterized in greatest possible detail.
At this stage, nanoLC-MS/MS provides for a detailed in-depth analysis of the injected sample with high sequence coverage. Information on post-translational modifications can be gained e.g. by creating neutral loss traces with regard to the dissociation of phosphate or glycosylation groups from the peptides in the MS/MS process.