Thermo Scientific™ ProPac™ SAX and WAX Protein Column by Thermo Fisher Scientific

High-Resolution, Strong and Weak Anion-Exchange Columns for the Separation of Proteins

The ProPac® SAX-10 is a nonporous, strong anion-exchange column (quaternary amine functional group). The ProPac WAX-10 column is a weak anion exchanger (tertiary amine functional group). Both are based on a nonporous core particle that provides pressure stability. A hydrophilic layer prevents unwanted secondary interactions and a grafted anion exchange surface provides pH control, selectivity, and fast mass transfer for high efficiency separation, as well as moderate capacity.

ProPac SAX-10 and WAX-10 columns can resolve protein isoforms that differ by as little as one charged residue. Differences in selectivity between the WAX-10 and SAX-10 provide flexibility in maximizing resolution of closely related species.


ProPac® SAX and WAX Protein Column Features:

• Unequalled resolution
• High-efficiency peaks with excellent recovery
• Higher capacity than other columns based on nonporous particles
• Useful for characterization and quality control assessment of closely-related protein variants
• Supports separation of proteins that differ by as little as one amino acid residue
• Neutral hydrophilic coat that eliminates protein-resin hydrophobic interactions
• Superior lot-to-lot and column-to-column reproducibility

Column Details:

• Length: 50, 250 mm
• ID: 2.0, 4.0, 9.0, 22 mm