ProLabel™ Expression and Cloning Vectors by DiscoveRx Corporation
Manufacturer DiscoveRx Corporation
Vectors for High-throughput Cellular Protein Trafficking Assays ProLabel™ Expression and Cloning Vectors offer convenient and flexible options for developing homogeneous, single-addition PathHunter™ screening assays to directly measure the effect of test compounds on protein translocation, secretion, or degradation by ß-galactosidase activity in whole mammalian cells. ProLabel Vectors can also be used to monitor general protein expression in a convenient antibody-free format. All ProLabel Vector... Read more
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ProLabel™ Expression and Cloning Vectors
Vectors for High-throughput Cellular Protein Trafficking Assays
ProLabel™ Expression and Cloning Vectors offer convenient and flexible options for developing homogeneous, single-addition PathHunter™ screening assays to directly measure the effect of test compounds on protein translocation, secretion, or degradation by ß-galactosidase activity in whole mammalian cells. ProLabel Vectors can also be used to monitor general protein expression in a convenient antibody-free format.
All ProLabel Vectors are designed with the 4-kDA ProLabel tag and the CMV promotor for constitutive protein expression in mammalian PathHunter EA-Nuc or in-house Cell Lines. If transfecting a ProLabel Vector into an EA-Nuc Cell Line for a PathHunter Assay, PathHunter Detection Reagents are purchased separately. For general protein expression assays, in-house cell lines may be used and EA must be added using EFC Detection Kits.
For membrane-based detection, expressed ProLabel-tagged proteins can be monitored using the fast, antibody-free EAstern Blot Assay Kit, or if ProLabel-c-myc vectors are used, by traditional Western blots.
Wide Selection of Vectors
ProLabel Expression Vectors are available with the ProLabel tag on a wide variety of proteins known to translocate to the nucleus. In addition, ProLabel Cloning Vectors are engineered for cloning a selected gene fused to the ProLabel tag into C1-3 or N1-3 frames.
Features and Benefits
» Fast, homogeneous procedure has no washes or centrifugation
» More sensitive than a Western blot or GFP
» Small ProLabel tag size minimizes protein function perturbation
PathHunter Assay Principle
When a ProLabel Vector is transfected into cells that express a large fragment of ß-gal in the nucleus (PathHunter EA-Nuc cells), translocation of the fusion protein to the nucleus enables two ß-gal framents to combine and generate signal when chemiluminescent substrate is present. In general non-PathHunter EFC assays, the EA protein is added as a reagent instead of nuclear expression.
Kit Contents
» Vector plasmid DNA
» Detection Kits
Instrument Requirements
» Luminometer, Multimode Microplate Reader or CCD Camera
ProLabel™ Expression and Cloning Vectors offer convenient and flexible options for developing homogeneous, single-addition PathHunter™ screening assays to directly measure the effect of test compounds on protein translocation, secretion, or degradation by ß-galactosidase activity in whole mammalian cells. ProLabel Vectors can also be used to monitor general protein expression in a convenient antibody-free format.
All ProLabel Vectors are designed with the 4-kDA ProLabel tag and the CMV promotor for constitutive protein expression in mammalian PathHunter EA-Nuc or in-house Cell Lines. If transfecting a ProLabel Vector into an EA-Nuc Cell Line for a PathHunter Assay, PathHunter Detection Reagents are purchased separately. For general protein expression assays, in-house cell lines may be used and EA must be added using EFC Detection Kits.
For membrane-based detection, expressed ProLabel-tagged proteins can be monitored using the fast, antibody-free EAstern Blot Assay Kit, or if ProLabel-c-myc vectors are used, by traditional Western blots.
Wide Selection of Vectors
ProLabel Expression Vectors are available with the ProLabel tag on a wide variety of proteins known to translocate to the nucleus. In addition, ProLabel Cloning Vectors are engineered for cloning a selected gene fused to the ProLabel tag into C1-3 or N1-3 frames.
Features and Benefits
» Fast, homogeneous procedure has no washes or centrifugation
» More sensitive than a Western blot or GFP
» Small ProLabel tag size minimizes protein function perturbation
PathHunter Assay Principle
When a ProLabel Vector is transfected into cells that express a large fragment of ß-gal in the nucleus (PathHunter EA-Nuc cells), translocation of the fusion protein to the nucleus enables two ß-gal framents to combine and generate signal when chemiluminescent substrate is present. In general non-PathHunter EFC assays, the EA protein is added as a reagent instead of nuclear expression.
Kit Contents
» Vector plasmid DNA
» Detection Kits
Instrument Requirements
» Luminometer, Multimode Microplate Reader or CCD Camera
Product Overview
ProLabel™ Expression and Cloning Vectors
Manufacturer DiscoveRx Corporation
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