ProcartaPlex™ Multiplex Immunoassays by Affymetrix, Inc.

ProcartaPlex Multiplex Immunoassays provide a reliable method for quantitating multiple analytes simultaneously with minimal time, cost and sample volume. ProcartaPlex assays produce consistent, highly correlative data when scaled to include more (or less) analytes.

The ProcartaPlex product line is developed and manufactured with the aim of producing multiplex bead-based assays that yield the same sensitivity, linearity, and dynamic range as that of traditional plate-based ELISAs.

ProcartaPlex Multiplex Immunoassays are available in multiple formats across six species (Human, Mouse, Rat, Non-Human Primate, Porcine and Canine) to meet the needs of your research.

ProcartaPlex Multiplex Immunoassays use Luminex® xMAP (multi-analyte profiling) technology that enables:

• Quantitate up to 50 analytes simultaneously
• Use significantly less sample (only 25-50 uL)
• Same amount of time that it takes to perform an ELISA

The xMAP technology applies to two bead types—magnetic and polystyrene. Both bead types are internally dyed with precise proportions of red and infrared fluorophores. The differing proportions of the red and infrared fluorophores result in 100 unique spectral signature microspheres which are identified by the Luminex xMAP detection systems. The conjugation of a distinct monoclonal antibody to a distinct bead allows for analysis of multiple analytes in a single well.