Focus on Your Results
Bio-Rad® PrimePCR™ assays for real-time PCR are expertly designed and wet-lab validated to ensure optimal assay performance. Bio-Rad offers a complete workflow solution for real-time PCR, including RNA isolation kits, reverse transcription kits, and real-time PCR supermixes, as well as real-time PCR detection systems and data analysis software.
Wet-Lab Validated for Guaranteed Performance
Wet-lab validation of every assay provides confidence in results while eliminating time-consuming assay design and optimization steps. Assays are validated for use with iScript™ advanced cDNA synthesis kit for RT-qPCR and SsoAdvanced™ SYBR® Green supermix for superior performance. Validation information is available for every assay
Assay Performance Standards
Sensitivity: Accurate detection of 20 copies
Specificity: Amplicon sequence validated with next generation sequencing (NGS); minimal primer-dimer formation and genomic DNA cross-reactivity
Amplification efficiency: 90–110%
Linear dynamic range: Minimum of six orders of magnitude; detection of a synthetic template standard curve from 20 to 20 million copies
Over 300 Pathway and Collection Panels
Predesigned plates are available for canonical pathways and related collections to help identify and investigate key targets in a biological pathway of interest. Predesigned plates can be modified to include user-selected assays.
Customizable 96- and 384-Well Plate Formats
Easy-to-use custom plate configurator allows users to lay out assays on a plate exactly as they choose, or to use a suggested template.
Fully Integrated with CFX Manager™ Software
Full integration with CFX real-time PCR detection systems and data analysis software streamlines data collection and analysis.
Application notes and news
- PrimePCR™ Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation
- Relative Quantitation of mRNA: Real-Time PCR vs. End-Point PCR
- PrimePCR™ Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy
- Bio-Rad Introduces New PrimePCR™ Mouse Assays and Panels — Industry’s Only Lab-Validated Real-Time PCR Prim...