Focus on Your Results
Bio-Rad® PrimePCR™ assays for real-time PCR are expertly designed and wet-lab validated to ensure optimal assay performance. Bio-Rad offers a complete workflow solution for real-time PCR, including RNA isolation kits, reverse transcription kits, and real-time PCR supermixes, as well as real-time PCR detection systems and data analysis software.

Wet-Lab Validated for Guaranteed Performance
Wet-lab validation of every assay provides confidence in results while eliminating time-consuming assay design and optimization steps. Assays are validated for use with iScript™ advanced cDNA synthesis kit for RT-qPCR and SsoAdvanced™ SYBR® Green supermix for superior performance. Validation information is available for every assay

Assay Performance Standards
Sensitivity: Accurate detection of 20 copies
Specificity: Amplicon sequence validated with next generation sequencing (NGS); minimal primer-dimer formation and genomic DNA cross-reactivity
Amplification efficiency: 90–110%
Linear dynamic range: Minimum of six orders of magnitude; detection of a synthetic template standard curve from 20 to 20 million copies
R²:  >0.99

Over 300 Pathway and Collection Panels
Predesigned plates are available for canonical pathways and related collections to help identify and investigate key targets in a biological pathway of interest. Predesigned plates can be modified to include user-selected assays.

Customizable 96- and 384-Well Plate Formats
Easy-to-use custom plate configurator allows users to lay out assays on a plate exactly as they choose, or to use a suggested template.

Fully Integrated with CFX Manager™ Software
Full integration with CFX real-time PCR detection systems and data analysis software streamlines data collection and analysis.