Diagenode’s Premium RRBS kit provides a powerful method to efficiently analyze DNA methylation without the higher costs associated with whole genome bisulfite sequencing.
By cutting the genome using the MspI enzyme (CCGG target sites) followed by size selection, DNA is enriched to represent CpG-rich regions (including CpG islands), in which DNA methylation marks are typically found. Thus, RRBS provides a method to more cost-effectively analyze DNA methylation by reducing the number of nucleotides that need to be sequenced and focusing on relevant CpG islands.
Diagenode’s premium RRBS technology: