Precision Plus Protein™ WesternC™ Blotting Standards, value pack, 50 applications by Bio-Rad

Use Precision Plus Protein WesternC Standards as a combination standard for western blotting and fluorescence detection in SDS-PAGE. These standards contain ten Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, and 75 kD), as well as unstained versions of the Strep-tagged recombinant proteins for molecular weight sizing after blot development.

Product Contents

1 - Precision Plus Protein WesternC Standards, 250 μl (1610376)

1 - Precision Protein™ StrepTactin-HRP Conjugate, 125 μl (1610381)

Applications and Uses

• Monitoring electrophoretic separation
• Monitoring blot transfer efficiency with easy orientation via multicolor standards
• Molecular weight sizing of proteins after blot development
• Fluorescent blot detection of prestained bands, with blue bands visualized in the red wavelength range and pink bands in the green wavelength range

More Information

Loading buffer: 30% (w/v) glycerol, 2% SDS, 62.5 mM Tris, pH 6.8, 50 mM DTT, 5 mM EDTA, 0.02% NaN₃, 0.01% bromophenol blue.

Fluorescent detection: prepare a 1:10 dilution of the WesternC Standard and load 5 µl per well. No conjugate is needed for fluorescent blot detection.

Related Products

• Precision Protein StrepTactin-HRP Conjugate, 300 µl (1610380)
• Precision Protein StrepTactin-HRP Conjugate, 125 µl (1610381)
• StrepTactin-AP Conjugate, 300 µl (1610382)

More Precision Plus Protein Standards:

• Precision Plus Protein Unstained Standards
• Precision Plus Protein Prestained Standards
• IEF and 2-D Electrophoresis Protein Standards

Related Categories

• Protein Ladders and Standards
• Precast Protein Gels
• Protein Electrophoresis and Blotting
• Western Blotting

Online Resources

• Protein Electrophoresis
• Western Blotting