Status:
Advanced ReviewerMember since: 2020
Organization: Boehringer-ingelheim, Inc
Good first choice screening column.
Application Area: API analysis in formulations
"Very reproducible results. For simple applications looking for a one peak of API, very good. They last long time. Not too good for high pH mobile phase, but Agilent has some substituted columns, which work fine with high pH."
Status:
ReviewerMember since: 2021
Organization: Elite Diagnostics
Great product, good customer service!
Application Area:HPLC
"It was very easy to use. The ordering process is super easy and sample arrived in timely manner."
Status:
Advanced ReviewerMember since: 2020
Organization: Boehringer-ingelheim, Inc
Poroshell C-18 columns - recommend them.
Application Area:Pre-clinical study analysis
"We use this type of column pretty much for all our pre-clinical analytical methods. They are good, reliable and give us reproducible results."
Status:
ReviewerMember since: 2020
Organization: LCGC
Best column for low pH applications.
Application Area:Pharmaceutical method development
"We haveseen better performance at low pH and with good chromatographic results."
Status:
ReviewerMember since: 2007
Organization: Mecachem
HILIC-Z without sharkfin-shaped peaks.
Application Area:HILIC-Z
"HILIC is not so easy to use as C18. The water layer has a lot of influence on a good separation and peak shape. In our facility we measure a lot of samples but they are never the same, so we often use generic methods and almost always with a gradient. These circumstances seldom gave good results on our previous HILIC columns (most of them were diol types), until we got the HILIC-Z column. Chromatography was easy, peak shapes were good (no sharkfin-shape!). Now we use the column in our walk-in facility, were chemist put in their own sample and use them often with nice results."
Status:
ReviewerMember since: 2020
Organization: Midwestern University College of Pharmacy-Glendale
Great columns. I will continue to purchase them.
Application Area:Metabolite analysis from animal plasma
"These columns are robust and the chromatography is consistent even after 100s of runs analyzing drugs/metabolites extracted from animal plasma."
Status:
Expert ReviewerMember since: 2019
Organization: University of the People (Health Science)
Will buy the same or similar brand again for amino acids. Ideal for long term use.
Application Area:Analyze vitamins in mineral premixes and water soluble powders
"Very good and clean separation of components, high theoretical plate count and easy to clean up. My only concern is pressures are up to 180 to 250 bar, which is almost or more than the capacity of our maximum pump pressure tolerance."
Status:
ReviewerMember since: 2016
Organization: Corium Internaitonal Inc.,
Highly recommend this column.
Application Area:Analyze small molecules in transdermal product
"The column is reproducible and reliable. I can use the column on both UPLC/UHPLC/HPLC. We got nice chromatograms with good separation with this column."
Status:
ReviewerMember since: 2018
Organization: MAPA GmbH
Good quality for low price
Application Area:BPA Analysis
"Good quality for this low price. The results are constant and the handling is easy."
Status:
ReviewerMember since: 2018
Organization: Mapa GmbH
Good part for us.
Application Area:BPA Analysis
"We need it for HPLC BPA Analysis. It works very well and we could make a new method with it."
Status:
Advanced ReviewerMember since: 2017
Organization: Raghva life science.
It is a good product
Application Area:separation and identification of impurities
"I would recommend to others for separation of impurities or identification of impurities"
Status:
ReviewerMember since: 2013
Organization: apotex.inc
Worth the time and money
Application Area:bioanalytical assays
"Poroshell 120 ec c18 is an analytical column, by Agilent, it is reliable, it has good performance and supports a lot of injections matrix or neat solutions "
Status:
ReviewerMember since: 2015
Organization: Global Chemicals (Thermo Fisher Scientific)
Poroshell 120 column is a Great product
Application Area:Separation of wide variety of analytes such as acidic, basic and neutral type of compounds
"I have been using Poroshell 120 column (50 mm x 2.1 mm x 2.7 micron) for more than two years now. This is a very efficient column that shows great resolution. Since I am using the LC-MS, the choice of mobile phase is limited for that. Never the less with that also I got good resolution and response."
Status:
ReviewerMember since: 2014
Organization: Pulmokine, Inc.
Application Area:HPLC-MS/MS Analysis
"I have been using this product for small molecule analysis of APIs. The advantage of the poroshell design is that it allows one to achieve high resolution on a traditional HPLC system. The ease of use is straightforward, just install the column, equilibrate and start separating. I have not received after sales care or inquires from Agilent. Value of the product is largely based on how many injections you can perform before column performance diminishes. I can typically get in the neighborhood of 1500-2500 injections of biological samples, before column replacement."
Status:
Advanced ReviewerMember since: 2008
Organization: -
Application Area:Pharmaceuticals; Drugs of Abuse
"This column is easy to use, and provided highly efficient separations. The column performance is robust even in high flow conditions. Because the column back-pressure is lower than other columns, it allows for higher flow applications such as UHPLC and/or high flow LC/MS applications."
Poroshell 120 columns provide rugged, high resolution separations within the pressure range of any mainstream LC system, making the benefits of sub-2 µm performance available with all existing LCs.
Poroshell columns can achieve high resolution and high speed separations on current instruments, and higher resolution and speeds on new high-pressure LC and LC/MS systems. Stable to 600 bar, Poroshell 120 are great for fast UHPLC analyses, and can accelerate your older, traditional 5 µm method for 4-5x reduction in analysis time. With a family of ten chemistries, including the recently introduced PFP, and alignment with the ZORBAX family chemistries, method development and transfer is flexible and easy.
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