Poroshell 120 by Agilent Technologies

4.6
/
5.0
  |  18 reviews
Poroshell 120 columns provide rugged, high resolution separations within the pressure range of any mainstream LC system, making the benefits of sub-2 µm performance available with all existing LCs.Poroshell columns can achieve high resolution and high speed separations on current instruments, and higher resolution and speeds on new high-pressure LC and LC/MS systems. Stable to 600 bar, Poroshell 120 are great for fast UHPLC analyses, and can accelerate your older, traditional 5 µm method for 4-5... Read more


Poroshell 120 by Agilent Technologies product image
Poroshell 120
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Average Rating: 4.6
18 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Advanced Reviewer
  • Member since: 2020

  • Organization: Boehringer-ingelheim, Inc



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Good first choice screening column.
Rating: 5.0

  • Application Area: API analysis in formulations

"Very reproducible results. For simple applications looking for a one peak of API, very good. They last long time. Not too good for high pH mobile phase, but Agilent has some substituted columns, which work fine with high pH."

Review date: 04 Feb 2022 | Poroshell 120
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Elite Diagnostics



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great product, good customer service!
Rating: 5.0

  • Application Area:HPLC

"It was very easy to use. The ordering process is super easy and sample arrived in timely manner."

Review date: 17 Nov 2021 | Poroshell 120
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: Harrens Lab



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Sharp peak with high efficiency.
Rating: 4.3

  • Application Area:Food, cannabis

"Good column, long life."

Review date: 23 Jul 2021 | Poroshell 120
  • Status:

    Advanced Reviewer

  • Member since: 2020

  • Organization: Boehringer-ingelheim, Inc



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Poroshell C-18 columns - recommend them.
Rating: 5.0

  • Application Area:Pre-clinical study analysis

"We use this type of column pretty much for all our pre-clinical analytical methods. They are good, reliable and give us reproducible results."

Review date: 16 Jul 2021 | Poroshell 120
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: LCGC



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Best column for low pH applications.
Rating: 5.0

  • Application Area:Pharmaceutical method development

"We haveseen better performance at low pH and with good chromatographic results."

Review date: 03 Mar 2021 | Poroshell 120
  • Status:

    Reviewer

  • Member since: 2007

  • Organization: Mecachem



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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HILIC-Z without sharkfin-shaped peaks.
Rating: 4.3

  • Application Area:HILIC-Z

"HILIC is not so easy to use as C18. The water layer has a lot of influence on a good separation and peak shape. In our facility we measure a lot of samples but they are never the same, so we often use generic methods and almost always with a gradient. These circumstances seldom gave good results on our previous HILIC columns (most of them were diol types), until we got the HILIC-Z column. Chromatography was easy, peak shapes were good (no sharkfin-shape!). Now we use the column in our walk-in facility, were chemist put in their own sample and use them often with nice results."

Review date: 13 Nov 2020 | Poroshell 120
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Midwestern University College of Pharmacy-Glendale



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great columns. I will continue to purchase them.
Rating: 5.0

  • Application Area:Metabolite analysis from animal plasma

"These columns are robust and the chromatography is consistent even after 100s of runs analyzing drugs/metabolites extracted from animal plasma."

Review date: 10 Jun 2020 | Poroshell 120
  • Status:

    Expert Reviewer

  • Member since: 2019

  • Organization: University of the People (Health Science)



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
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Will buy the same or similar brand again for amino acids. Ideal for long term use.
Rating: 3.7

  • Application Area:Analyze vitamins in mineral premixes and water soluble powders

"Very good and clean separation of components, high theoretical plate count and easy to clean up. My only concern is pressures are up to 180 to 250 bar, which is almost or more than the capacity of our maximum pump pressure tolerance."

Review date: 06 Jun 2020 | Poroshell 120
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Corium Internaitonal Inc.,



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Highly recommend this column.
Rating: 4.7

  • Application Area:Analyze small molecules in transdermal product

"The column is reproducible and reliable. I can use the column on both UPLC/UHPLC/HPLC. We got nice chromatograms with good separation with this column."

Review date: 26 Jul 2018 | Poroshell 120
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: MAPA GmbH



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Good quality for low price
Rating: 5.0

  • Application Area:BPA Analysis

"Good quality for this low price. The results are constant and the handling is easy."

Review date: 23 Feb 2018 | Poroshell 120


  • Status:

    Reviewer

  • Member since: 2018

  • Organization: Mapa GmbH



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Good part for us.
Rating: 4.3

  • Application Area:BPA Analysis

"We need it for HPLC BPA Analysis. It works very well and we could make a new method with it."

Review date: 23 Feb 2018 | Poroshell 120
  • Status:

    Advanced Reviewer

  • Member since: 2017

  • Organization: Raghva life science.



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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It is a good product
Rating: 4.7

  • Application Area:separation and identification of impurities

"I would recommend to others for separation of impurities or identification of impurities"

Review date: 08 Feb 2017 | Poroshell 120
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: apotex.inc



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Worth the time and money
Rating: 5.0

  • Application Area:bioanalytical assays

"Poroshell 120 ec c18 is an analytical column, by Agilent, it is reliable, it has good performance and supports a lot of injections matrix or neat solutions "

Review date: 24 May 2016 | Poroshell 120
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Global Chemicals (Thermo Fisher Scientific)



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Poroshell 120 column is a Great product
Rating: 5.0

  • Application Area:Separation of wide variety of analytes such as acidic, basic and neutral type of compounds

"I have been using Poroshell 120 column (50 mm x 2.1 mm x 2.7 micron) for more than two years now. This is a very efficient column that shows great resolution. Since I am using the LC-MS, the choice of mobile phase is limited for that. Never the less with that also I got good resolution and response."

Review date: 04 Apr 2016 | Poroshell 120
  • Status:

    Reviewer

  • Member since: 2014

  • Organization: Pulmokine, Inc.



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
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Rating: 4.0

  • Application Area:HPLC-MS/MS Analysis

"I have been using this product for small molecule analysis of APIs. The advantage of the poroshell design is that it allows one to achieve high resolution on a traditional HPLC system. The ease of use is straightforward, just install the column, equilibrate and start separating. I have not received after sales care or inquires from Agilent. Value of the product is largely based on how many injections you can perform before column performance diminishes. I can typically get in the neighborhood of 1500-2500 injections of biological samples, before column replacement."

Review date: 18 Feb 2015 | Poroshell 120
  • Status:

    Advanced Reviewer

  • Member since: 2008

  • Organization: -



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Rating: 4.3

  • Application Area:Pharmaceuticals; Drugs of Abuse

"This column is easy to use, and provided highly efficient separations. The column performance is robust even in high flow conditions. Because the column back-pressure is lower than other columns, it allows for higher flow applications such as UHPLC and/or high flow LC/MS applications."

Review date: 13 Oct 2014 | Poroshell 120
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: Universidad San Pablo



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    3 out of 5
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Rating: 4.3
"High efficiency, high price."

Review date: 31 Aug 2011 | Poroshell 120
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: Merck



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Rating: 5.0
"great retention and peak shape for basic small molecule pharmaceuticals."

Review date: 13 Jun 2011 | Poroshell 120

Poroshell 120 columns provide rugged, high resolution separations within the pressure range of any mainstream LC system, making the benefits of sub-2 µm performance available with all existing LCs.

Poroshell columns can achieve high resolution and high speed separations on current instruments, and higher resolution and speeds on new high-pressure LC and LC/MS systems. Stable to 600 bar, Poroshell 120 are great for fast UHPLC analyses, and can accelerate your older, traditional 5 µm method for 4-5x reduction in analysis time. With a family of ten chemistries, including the recently introduced PFP, and alignment with the ZORBAX family chemistries, method development and transfer is flexible and easy.


Features:

  • High efficiency and high resolution, with up to 50% less backpressure than sub-2 μm columns
  • 2 μm frit, for rugged performance with dirty samples
  • Compatible with 400 bar and 600 bar LCs, as well as UHPLC instruments
  • An expanding family of bonded phases to align with the ZORBAX Family, for reliable scalability
  • Excellent selectivity and peak shapes
  • Designed for exceptional reproducibility
  • Explore Poroshell 120 solutions focused applications, from food to pharma

Product Overview

Poroshell 120 by Agilent Technologies product image

Poroshell 120

Manufacturer Agilent Technologies

4.6 / 5.0 | 18 reviews