For the isolation and enrichment of polySUMOylated proteins.
1. Capture and isolation of polySUMOylated protein conjugates from cell/tissue lysates of interest with subsequent detection and analysis by Western blotting.
2. Biochemical characterisation, secondary purification by substrate-specific immunoprecipitation, or identification of polySUMO modified proteins by proteomic analysis following release of free polySUMOylated proteins in their active/native form by specific elution from the affinity matrix,.
3. Selective purification/pull down of polySUMOylated proteins from in vitro SUMOylation assays.