PME Free-Circulating DNA Extraction Kit
|Vijay Kumar Kuna|
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Member since 2014
Laboratory of Transplantation and Regenerative Medicine
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"The product is easy to use because of its easy procedure requiring less equipment and not very time consuming to obtain final results. I would recommend my colleagues to use this kit in their research. All the doubts were clearly answered by the company representatives after sending the kit. Even though I feel the kit is a bit expensive, I feel this will help in my research. The kit is very sensitive. "
New Polymer Mediated Enrichment (PME) technology for free circulating DNA extraction
Circulating cell-free DNA is a very interesting diagnostic target, but the amount of free-circulating DNA is usually very low and varies among different individuals. Further, these nucleic acids are present as short fragments, typically smaller than 1000 nt, making the efficient extraction process challenging. Because of the high sample volumes the protocols of commercially available kits are very labor-intensive as well as time consuming and need a lot of reagents.
The PME free-circulating DNA Extraction Kit is based on a new, patent-pending technology called PME – Polymer Mediated Enrichment. As first step cell-free DNA in the entire sample is captured by a polymer. Afterwards this complex is collected as a pellet by centrifugation. Subsequently the captured nucleic acid is dissolved in a special buffer thus reducing the sample volume in the following extraction significantly.
PME Free-Circulating DNA Extraction Kit Features:
- Easy handling, high efficiency and extremely time saving
- Processing of starting sample volumes up to 5 ml
- Proven for serum and plasma from different blood collection systems
- Novel, patent pending technology: Polymer Mediated Enrichment (PME)
- Enrichment & extraction in approx. 30 min from 1 ml or approx. 1 h from 5 ml of serum or plasma and up to 10 ml from urine