PME Free-Circulating DNA Extraction Kit and DNA Kit-IPC16

PME Free-Circulating DNA Extraction Kit.

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PME Free-Circulating DNA Extraction Kit and DNA Kit-IPC16
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Features of manual nucleic acid isolation: Easy handling, high efficiency and extremely time saving Processing of starting sample volumes up to 10 ml Proven for serum and plasma from different blood collection systems Novel, patent pending technology: PME – Polymer Mediated Enrichment Enrichment & extraction in approx. 30 min from 1 ml or approx. 1 h from 5 ml of serum or plasma and up to 10 ml from urine Features of automated nucleic acid extraction: Automized extraction of free-circulating DNA Processing of starting sample volumes up to 10 ml Novel, patent pending technology: PME – Polymer Mediated Enrichment Enrichment & extraction in approx. 76 minutes from 1 ml or approx. 1,5 h from 5 ml of serum or plasma and up to 10 ml from urine ...Read more

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Reviews

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Average Rating: 4.3

Ease of use
4 out of 5
After sales service
5 out of 5
Value for money
4 out of 5
Rating: 4.3
Ease of use
4 out of 5
After sales service
5 out of 5
Value for money
4 out of 5
  • Review date: 29 May 2014

  • Application Area: Tissue Engineering

  • PME Free-Circulating DNA Extraction Kit and DNA Kit-IPC16

"The product is easy to use because of its easy procedure requiring less equipment and not very time consuming to obtain final results. I would recommend my colleagues to use this kit in their research. All the doubts were clearly answered by the company representatives after sending the kit. Even though I feel the kit is a bit expensive, I feel this will help in my research. The kit is very sensitive. "

Description

Features of manual nucleic acid isolation:

  • Easy handling, high efficiency and extremely time saving
  • Processing of starting sample volumes up to 10 ml
  • Proven for serum and plasma from different blood collection systems
  • Novel, patent pending technology: PME – Polymer Mediated Enrichment
  • Enrichment & extraction in approx. 30 min from 1 ml or approx. 1 h from 5 ml of serum or plasma and up to 10 ml from urine

Features of automated nucleic acid extraction:

  • Automized extraction of free-circulating DNA
  • Processing of starting sample volumes up to 10 ml
  • Novel, patent pending technology: PME – Polymer Mediated Enrichment
  • Enrichment & extraction in approx. 76 minutes from 1 ml or approx. 1,5 h from 5 ml of serum or plasma and up to 10 ml from urine
  • For usage of InnuPure® C16 and up to 16 samples in parallel

 

Circulating cell-free DNA is a very interesting diagnostic target, but the amount of freecirculating DNA is usually very low and varies among different individuals. Further, these nucleic acids are present as short fragments, typically smaller than 1000 nt, making the efficient extraction process challenging. Because of the high sample volumes the protocols of commercially available kits are very labor-intensive as well as time consuming and need a lot of reagents.

The PME free-circulating DNA Kit-IPC16 is based on a new, patent-pending technology called PME – Polymer Mediated Enrichment. As first step cell-free DNA in the entire sample is captured by a polymer. Afterwards this complex is collected as a pellet by centrifugation. Subsequently the captured nucleic acid is dissolved in a special buffer thus reducing the sample volume in the following extraction significantly. Subsequently the extraction of free-circulating DNA from up to 16 samples in parallel takes place within InnuPure® C16.

 

Applications:

• Enrichment and isolation of cell-free DNA from serum, plasma or urine
• Sample preparation for further downstream-applications in pre-natal diagnostics, the diagnosis of metabolic diseases, or sports medicine

Product Overview

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PME Free-Circulating DNA Extraction Kit and DNA Kit-IPC16

by Analytik Jena Life Science | Available Worldwide

4.3/5.0 (1 reviews)
Ease of use
4 out of 5
After sales service
5 out of 5
Value for money
4 out of 5






Receive your quote directly from Analytik Jena Life Science for PME Free-Circulating DNA Extraction Kit and DNA Kit-IPC16