Platinum® Pfx DNA Polymerase is ideal for amplification of DNA fragments for high-fidelity PCR applications. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA Polymerase from Thermococcus species KOD with proofreading (3´a5´ exonuclease) activity. Platinum® antibody technology provides a simple, automatic hot-start method that improves PCR specificity (2,3) (Figure 1). PCRx Enhancer Solution is included for higher primer specificity, broader magnesium concentration, broader annealing temperature, and improved thermostability of Platinum® Pfx DNA Polymerase. The PCRx Enhancer Solution also helps optimize PCR of problematic and/or GC-rich templates.

Platinum® Pfx provides:

• 26 times higher fidelity than Taq DNA polymerase (1)
• Amplification of fragments up to 12 kb
• Room temperature reaction assembly

Application:

Amplification of DNA from complex View Moregenomic, viral, and plasmid templates; and RT-PCR (1). Platinum® Pfx is especially effective where the very high levels of fidelity are required.