Overview PhosphoQuest Enzyme Immunometric Assay (EIA) kits are complete kits for the quantitative determintation of phospho-kinases in cell lysates.
Features and Benefits » Measure cell based kinases » Stable, color coded liquid reagents
Cell based kinases using PhosphoQuest PhosphoQuest Assays use monoclonal antibodies which are immobilized on a microtiter plate to bind the phosphorylated specific protein as a standard or a sample. After a short incubation the excess sample or standard is washed out and a rabbit polyclonal antibody to phosphophorylated specific protein is added. This antibody binds to the phosphorylated specific protein captured on the plate. After a short incubation the excess antibody is washed out and goat anti-rabbit IgG conjugated to Horseradish peroxidase is added, which binds to the polyclonal phospho-antibody. Excess conjugate is washed out and substrate is added. After a short incubation, the enzyme reaction is stopped and the color generated is read at 450 nm. The measured optical density is directly proportional to the concentration of phospho-protein in either standards or samples.
Kit Contents » 96 strip-well plate coated with a monoclonal antibody » Phospho-kinase specific antibody » Phospho-kinase specific conjugate » Assay Buffer » Wash Buffer » Standards
Instrument Requirements » Absorbance
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