PhiX Control v3 is a reliable, adapter-ligated library used as a control for Illumina sequencing runs. The library is derived from the small, well-characterized PhiX genome, offering several benefits for sequencing and alignment.
It can be rapidly aligned for estimating relevant SBS metrics such as phasing, prephasing, and error rate. Depending on the application PhiX Control v3 can be used as a high-level spike in for unbalanced (CG or AT biased) samples, a low-level spike in, or as a dedicated control lane alongside low diversity samples. It is also an excellent control for troubleshooting cluster generation problems, allowing you to quickly determine if an error is related to sample preparation. The versatile PhiX Control v3 can be used in diverse applications to add value to your workflow and increase confidence in your results.