phi29 DNA Polymerase is the replicative polymerase from the Bacillus subtilis phage phi29 (Φ29). This polymerase has exceptional strand displacement and processive synthesis properties. The polymerase has an inherent 3´→5´ proofreading exonuclease activity.
An E. coli strain that carries the phi29 DNA Polymerase gene from bacteriophage phi29
•Replication requiring a high degree of strand displacement and/or processive synthesis
•High fidelity replication at moderate temperatures