Anomalous scattering with soft X-ray radiation creates new possibilities in phasing for macromolecular crystallography. The heart of the Phasing HomeLab™ is the FR-E DW SuperBright™ generator, the only microfocus rotating anode laboratory X-ray source featuring controlled wavelength selection. The FR-E DW SuperBright provides a cost-effective path to enhanced homelab single-wavelength anomalous diffraction (SAD) phasing while retaining traditional data collection capabilities. Other Rigaku rotating anode generators can also be equipped with Chromium (Cr) targets to provide the foundation for a Phasing HomeLab. All Phasing HomeLabs use the same optics and choice of detector. Osmic™ VariMax™ Cr optics maximize the beam produced by the Rigaku generators and deliver it exactly where it is needed: on the crystal. Incident and exit helium beam paths reduce air absorption and reduce background scatter. Since Cr radiation expands the diffraction pattern relative to Cu, a large aperture detector, such as the Rigaku R-AXIS IV++ , R-AXIS HTC or R-AXIS HR is recommended. The X-stream™ 2000 low temperature system maintains the crystals at cryogenic temperatures without the need for a house supply of liquid nitrogen. Since Cr radiation expands the diffraction pattern relative to Cu, a large aperture detector, such as the Rigaku R-AXIS IV++ or R-AXIS HTC is recommended.
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