Thermo Scientific™ pH Gradient Buffer Solutions by Thermo Fisher Scientific

Traditionally cation-exchange chromatography using salt gradients has been successfully used to characterize MAb charge variants. However, additional effort is often required to tailor the salt gradient method for individual charge variants. Thermo Scientific™ pH buffer solutions and kits can be used to generate highly reproducible, linear pH gradients using cation-exchange chromatography.

This generic, LC-based platform approach saves time in method development and facilitates method transfer to QA/QC for a wide range of MAb charge variants. Unlike traditional salt gradients, it is possible to predict the pI and the expected retention of the charge variants and use a narrow pH range to get a higher resolution separation.

pH Gradient Buffer Solutions Features:

• Proprietary buffer formulations enable fast,robust and reproducible pH gradients that are simple to optimize and easily automated
• Ready to usewith existing LC columns and systems, without the need for time-consuming mobile phase adjustments
• Applicable to the majority of MAbs