pGEM®-T Easy Vector Systems by Promega Corp.

"The pGEM-T Easy vector is linearized with - A single 3´-terminal thymidine at both ends. - The T-overhangs at the insertion sites improve the efficiency of ligation - Insertional inactivation of the α-peptide allows identification of recombinants by blue/white screening on indicator plates. - Contain numerous restriction sites within the multiple cloning region. - Multiple cloning region is flanked by recognition sites for the restriction enzymes - A double-digestion may be used to release the insert from either vector. - An overnight incubation at 4°C produces the maximum number of transformants"

"Reliable system for molecular cloning that works every time. Highly recommended."

"The pGEM-T easy vector system is one of the best products we use for cloning. It has high efficiency for the genes we work with and purchase the system with the competent cells as we are guaranteed results. The step by step protocol is easy to follow and is quick. We have also managed to reproduce the results and use the same methods for many genes."

"Very easy to use, clear information and instructions are super easy to follow. Gave exactly the results that were expected, and very simple to use to check PCR products. Would not use any other product."

"The pGEM-T easy vector system is one of the best product in cloning with high efficiency. I prefer to buy them along with competent cells, even 1/4 of the recommended amount of competent cells works well. I tried many cloning kits but feel this is the best one in term of repeatability of results"

"This is really the product which has high efficiency and reproducibility. I suggest buying it alongside competent cells. It works great with very little chance of calibration"