Status:
Advanced ReviewerMember since: 2019
Organization: University of Wolverhampton
Great results
Application Area: Cancer Research
"Very easy cloning system with reproducible results"
Status:
ReviewerMember since: 2022
Organization: Saga University
Using the product on a daily basis because of its best performance.
Application Area:Vector construction for gene isolation.
"The pGEM-T Easy vector is linearized with - A single 3´-terminal thymidine at both ends. - The T-overhangs at the insertion sites improve the efficiency of ligation - Insertional inactivation of the α-peptide allows identification of recombinants by blue/white screening on indicator plates. - Contain numerous restriction sites within the multiple cloning region. - Multiple cloning region is flanked by recognition sites for the restriction enzymes - A double-digestion may be used to release the insert from either vector. - An overnight incubation at 4°C produces the maximum number of transformants"
Status:
Advanced ReviewerMember since: 2019
Organization: University of Wolverhampton
Great product.
Application Area:Molecular biology
"Reliable system for molecular cloning that works every time. Highly recommended."
Status:
Advanced ReviewerMember since: 2019
Organization: University of Wolverhampton
Our 'go to' for cloning
Application Area:Cloning metastasis genes for expression and sequencing analysis
"The pGEM-T easy vector system is one of the best products we use for cloning. It has high efficiency for the genes we work with and purchase the system with the competent cells as we are guaranteed results. The step by step protocol is easy to follow and is quick. We have also managed to reproduce the results and use the same methods for many genes."
Status:
ReviewerMember since: 2019
Organization: University of Cambridge
Great result, very quick and easy to use.
Application Area:Checking PCR
"Very easy to use, clear information and instructions are super easy to follow. Gave exactly the results that were expected, and very simple to use to check PCR products. Would not use any other product."
Status:
Expert ReviewerMember since: 2017
Organization: Punjab Agricultural University
Great product in cloning
Application Area:Cloning and Sequencing
"The pGEM-T easy vector system is one of the best product in cloning with high efficiency. I prefer to buy them along with competent cells, even 1/4 of the recommended amount of competent cells works well. I tried many cloning kits but feel this is the best one in term of repeatability of results"
Status:
Expert ReviewerMember since: 2017
Organization: Punjab Agricultural University
I am fond of this product
Application Area:Cloning and sequencing
"This is really the product which has high efficiency and reproducibility. I suggest buying it alongside competent cells. It works great with very little chance of calibration"
The pGEM®-T Easy Vector Systems are convienent systems to clone PCR products.
They offer all of the advantages of the pGEM®-T Vector Systems with added convieneice of recognition sites for EcoRI and NotI flankin the insertion site. Thus, several options exist to remove the desired insert DNA with a single restriction digestion. The pGEM(R)-T Easy Vectr System II contains JM109 Competent Cells in addition to all pf the pGEM(R)-T Easy Vector System I components.
pGEM®-T Easy Vector Systems Features & Benefits: