The pET E. coli expression system is a widely used in vivo bacterial expression system due to the strong selectivity of the bacteriophage T7 RNA polymerase for its cognate promoter sequences, the high level of activity of the polymerase, and the high efficiency of translation mediated by the T7 gene.
- Tightly controlled T7 RNA polymerase-based gene expression in E. coli
- High-level expression upon induction
- Convenient cloning sites for optimal expression of cloned protein-coding sequence
- BL21(DE3) and BL21(DE3)pLysS competent cells are provided