Average Rating: 5.0
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"Glow discharge of carbon-coated TEM grids is a mandatory procedure to successfully prepare nice negatively stained or cryo-TEM specimens. Previously I was using a homemade glow-discharge device that worked rather well, however it was deemed not safe enough and risk free. Therefore, I purchased the easiGlow system, it consists of a small compact unit and a rotary pump. The system is very easy to use and the procedure is automatic (you can modify several parameters though). You just have to press one button and a couple of minutes later the grids are glow discharged. It's a very practical system for a mulit-user environment."Review date:
17 Oct 2012 | PELCO easiGlow™ Glow Discharge Cleaning System
For one of the most common glow discharge applications, making TEM support films or grids hydrophilic using air, the PELCO easiGlow™ uses an automated and quick cycle with fully selectable parameters. The microprocessor controlled system also offers full manual control for all parameters and an advanced protocol programming feature for custom designed glow discharge applications. Parameters and protocols can be stored to facilitate consistent glow discharge results. Open the shipping box, plug it in, and you can start.
The PELCO easiGlow™ Glow Discharge Cleaning System is a compact, quick and easy to use standalone system. It is primarily designed for cleaning TEM grids and hydrophilization of TEM carbon support films, which have the tendency to be hydrophobic. A glow discharge treatment with air will make a carbon film surface negatively charged (hydrophilic) which allows aqueous solutions to spread easily.
With increasingly demanding imaging applications, using clean and consistent quality TEM grids and supports has become more important than ever. Glow discharge treatment of TEM grids removes adsorbed hydro carbons, cleaning them while making them hydrophilic. The PELCO easiGlow™ is an affordable and easy to operate glow discharge system for any TEM lab.
The practical chamber size with its newly designed glow discharge head and adjustable stage with glass slide holder allows for easy loading and fast turnaround times. The system is fully microprocessor controlled and includes an intelligent touch screen device for control and display of operation parameters. The PELCO easiGlow™ supports both hydrophilic and hydrophobic treatment with either negative or positive charge and includes two separately controlled gas inlets. The required vacuum level is set by an electronically controlled precision proportional valve. This novel approach eliminates manual setting with a needle valve. A gentle venting procedure ensures that the TEM grids are not disturbed when the system is vented.