pDual GC Vector 20 ug (1 ug/ul) by Agilent Technologies

The pDual Expression Vector offers high-level heterologous gene expression in both mammalian and prokaryotic systems. A special feature of the pDual vector is the tandemly arranged bacterial Shine-Dalgarno and mammalian Kozak consensus sequence. Each ribosome binding site is positioned at its optimal distance from the initiation codon of the target gene for the highest translational efficiency in either mammalian or bacterial cells. The vector also features a calmodulin-binding peptide (CBP) tag which can be used for affinity purification and identification by Western blot.

The pDual GC Vector offers a C-terminal tag with three copies of the human c-Myc epitope tag (one copy is EQKLISEEDL) and a single copy of the 6xHis purification tag. The c-Myc epitope and 6xHis purification tags, expressed on the C-terminus of the protein, can be used for easy detection and purification of the fusion protein from mammalian and bacterial cell lysates. Use of the c-Myc epitope and the 6xHis purification tags allows every target gene to be detected and purified using the exact same protocol and reagents, saving you time and money.

For constitutive expression in mammalian cells, the pDual expression vector contains a mutagenized version of the CMV promoter/enhancer. Inducible gene expression in prokaryotes is directed from the hybrid T7/lacO promoter.

Features:

• Enables heterologous gene expression and high-level protein expression in both mammalian and prokaryotic systems.
• Eliminates the need to subclone.
• Express protein as either a native or tagged fusion protein.
• Features various tags including the calmodulin-binding peptide (CBP) tag, c-Myc epitope and 6xHis purification tags.

For Research Use Only. Not for use in diagnostic procedures.