A chimeric intron was inserted between the promoter and PCSK 9 cDNA to enhance expression. The expression cassette was flanked by a 1.2 kb insulator sequence from the chicken beta-globulin gene at both the 5’ and 3’ ends of the transgene. The expression construct was injected into the pronucleus of fertilized eggs from C57BL6J/N mice and the resulting founders and their transgenic offspring were identified from tail tissue by a PCR product amplified from the insulator sequence. Transferred to Charles River under exclusive license from Pfizer Inc., in 2011.
Model Properties: