PCR Optimization Kits by Agilent Technologies

10X Reaction Buffers

Deoxynucleotide Mix, PCR Grade

PCR Polishing Kit

Perfect Match PCR Enhancer

Taq Extender PCR Additive  

10X Reaction Buffers
These reactions buffers contain the optimal magnesium concentration for efficient PCR.

• Ideal for optimal PCR performance
• Standard 10X formulation
• Optimized for use with specific PCR enzymes

Deoxynucleotide Mix, PCR Grade
The PCR grade deoxynucleotide mix includes 25 mM of each standard dNTP (GATC) for use with any PCR application, including real-time quantitative PCR.

• Produces high-quality reaction products
• Withstands multiple freeze-thaw cycles without compromising efficiency
• An essential component of primer extension reactions

PCR Polishing Kit
The PCR Polishing Kit is designed to increase the blunt-ended cloning efficiencies associated with polymerase chain reaction (PCR)-generated fragments.

• Optimizes blunt-end cloning efficiencies associated with PCR-generated fragments
• Generates blunt-ended DNA fragments in 30 minutes
• Optimizes the PCR-Script cloning method
• More efficient than Klenow polishing

Perfect Match PCR Enhancer
Perfect Match PCR enhancer is an additive that increases the specificity and yield of primary PCR amplification products. When added to genomic in vitro amplification reactions, Perfect Match PCR enhancer destabilizes many mismatched primer-template complexes that would otherwise result in a heterogeneous population of amplified molecules.

• Increases yield and specificity of primary PCR amplification products
• Minimizes formation of poorly matched primer-template complexes
• Add to genomic PCR reactions to destabilize many mismatched primer-template complexes

Taq Extender PCR Additive
Taq Extender PCR additive improves the reliability and yield of conventional Taq-based PCR amplifications. Taq Extender additive increases the efficiency of Taq DNA polymerase extension reactions during each cycle of PCR by reducing mismatch pausing, resulting in a greater percentage of completed extension reactions.

• Improves length, yield and reliability of PCR
• Enhances efficiency of template extension by Taq DNA polymerase
• Improves amplification of difficult targets