The pBC vectors were derived from the pBluescript II phagemid. The ampicillin-resistance gene has been replaced with the chloramphenicol resistance gene. pBC phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping. Convenient selection when subcloning from ampicillin-resistant cloning vectors. Derived by replacing ampicillin-resistance gene of original pBluescript II vector with chloramphenicol-resistance gene. Large and versatile polylinker in two orientations for cloning flexibility. Available with f1 origin in either (+) or (__) orientation Convenient selectio...Read more
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