The PathDetect in vivo signal transduction pathway trans-reporting systems are designed for specific, rapid, and convenient assessment of the in vivo activation of transcription activators and upstream signal transduction pathways. The systems are useful for identifying whether a newly cloned gene is involved in a signal transduction pathway, and if so, at which step of the pathway.
Identify specific stimuli that leads to the phosphorylation of a test transcription factor
Easier and faster than Western blotting or gel-shift assays
Sensitive, quantitative assays
Stable cell lines available for constitutive expression of CREB, Elk1, and CHOP
PathDetect pFC2-dbd Trans-Reporter Control Plasmid