PathDetect Cis-Reporting Systems

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The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element.

  • Identify pathway-specific transcription activation
  • Easier, faster than Western blotting or gel-shift assays
  • Sensitive, quantitative assays
  • Antibodies now available
  • Stable cell lines available for constitutive expression of CREB, Elk1, c-Jun, and CHOP
  • PathDetect Trans-Reporting Systems -Identify specific stimuli that leads to the phosphorylation of a test transcription factor