PathDetect Cis-Reporting Systems by Agilent Technologies

Determine if a gene product or compound activates pathways leading to specific enhancers with our PathDetect Cis-Reporting Systems.  When PathDetect cis-reporting and expression plasmids encoding your gene of interest are co-transfected into mammalian cells, increased reporter activity indicates transcription activation and involvement of your gene product in the pathway leading to the enhancer element  


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PathDetect Cis-Reporting Systems
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Determine if a gene product or compound activates pathways leading to specific enhancers with our PathDetect Cis-Reporting Systems.  When PathDetect cis-reporting and expression plasmids encoding your gene of interest are co-transfected into mammalian cells, increased reporter activity indicates transcription activation and involvement of your gene product in the pathway leading to the enhancer element

For ultimate flexibility, our pLuc-MCS expression vector includes a multiple cloning site to facilitate insertion of your enhancer or transcription element. The system also includes positive and negative controls that ensure the success of your experiments.

  • Identify pathway-specific transcription activation
  • Easier and faster than blotting or gel-shift assays
  • Sensitive, quantitative assays

For Research Use Only. Not for use in diagnostic procedures