pAD-GAL4 Undigested Vector Kit

pAD-GAL4 Undigested Vector Kit

Agilent Technologies

The HybriZAP-2.1 two-hybrid vector system, a eukaryotic system to detect proteinprotein interactions in vivo , provides a method for the rapid identification of genes encoding proteins that interact ...read more

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Description

 

The HybriZAP-2.1 two-hybrid vector system, a eukaryotic system to detect proteinprotein interactions in vivo, provides a method for the rapid identification of genes encoding proteins that interact with a bait protein. The system is based on the ability to separate eukaryotic transcriptional activators into two separate domains, the DNA binding domain (BD) and the transcriptional activation domain (AD).

  •  Detects protein-protein interactions in vivo using the yeast GAL4 two-hybrid system
  •  Uses a lambda vector for generating high-efficiency cDNA and genomic libraries with high percentage of inserts and increased representation
  •  Easily converts to plasmid format for library screening by in vivo mass excision3

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