A CE-based system for the analysis of nucleic acids. The system provides clear advantages over traditional electrophoresis for DNA quantitation (viral load, gene expression), mutation analysis, and size polymorphisms analysis (VNTR, STR, microsatellites, RFLP, RAPD). System includes a P/ACE MDQ configured with a dual-wavelength laser-induced fluorescence detector, 488 nm argon ion laser module, an ambient sample storage module, and 32 Karat™ Software configured on an IBM personal computer. Installation Qualification, Operation Qualification 1 (OQ1) and documentation to aid in software validation is also included.
Nucleic Acid Analysis
The high resolving power of capillary gel electrophoresis (CGE) and direct UV detection allow the separation and quantitation of oligonucleotides with single base resolution. Illustrated is the separation of a 45-mer oligonucleotide, with clear resolution of the n-1 product.
Analysis in solution allows one to easily define an environment to study the interactions of proteins with nucleic acids. Whether research on transcription factors or studies measuring the kinetics of complexation, The P/ACE™ MDQ Molecular Characterization system provides a platform to assist you in performing these experiments.
Quantitation of Gene Expression
Capillary gel electrophoresis with LIF detection accurately quantitates the target and competitor PCR* product. Because the separations of these sequences are based on size, confirmation of fragment identity is achieved.
Solutions for the Analysis of Macromolecules
This CD-ROM tutorial provides seminars on the development of solution-based assays for nucleic acid, protein and carbohydrate analyses. Animation describing these analytical processes is shown, along with practical demonstrations on the implementation of these assays. Our focus is to provide you with tools that improve productivity by cutting through the complexity with simple, bold analytical solutions.
Agarose gel electrophoresis (AGE) has been the primary method used to assess the homogeneity of plasmid DNA, however, this approach has some major disadvantages. The AGE method is manual, only semi-quantitative and the assignment of bands to plasmid structures is difficult, as the electrophoretic mobility of plasmids of different shapes changes with the electrophoresis operating conditions A more powerful routine technology for the quantification of plasmid forms is capillary gel electrophoresis (CGE). This automated approach offers high resolution, high sensitivity and high reproducibility to this analysis.
Quantitation by Direct Hybridization
CE-LIF provides the sensitivity required for direct hybridization analysis in solution. DNA-probe complexes can be detected at very low levels, removing the need for PCR amplification. Separation and quantitation of complexes from unbound probes is typically achieved in less than 20 minutes.