Oris Cell Migration Assay – TriCoated (5 Pack) by AMSBIO

The Oris™ Cell Migration Assay – TriCoated is a reproducible, sensitive, and flexible assay that can be used to monitor cell migration on three differently coated surfaces. Optimizing conditions for conducting cell migration studies can be costly and time consuming. The new Oris™ Cell Migration Assay - TriCoated combines three kits into one for evaluating the best surface coating for your cell migration application. Formatted for a 96-well plate, the assay utilizes Oris™ Cell Seeding Stoppers made from a medical-grade silicone to restrict cell seeding to the outer annular regions of the wells. Removal of the stoppers reveals a 2mm diameter unseeded region in the center of each well, i.e., the detection zone, into which the seeded cells may then migrate. The Oris™ Detection Mask is applied to the plate bottom and restricts visualization to the detection zones, allowing only cells that have migrated to be detected (see Figure 1). The Oris™ Cell Migration Assay – TriCoated provides wells that are Tissue Culture Treated, Collagen I coated and Fibronectin coated (see Figure 2). The kit is designed to be used with any commercially available stain or labeling technique. Readout can be performed by microscopy or use of a microplate reader. The Oris™ Cell Migration Assay – TriCoated system has been designed for use with adherent cell cultures. This assay has been successfully used with HT-1080, PC-3, A549, NCI H1650, MDA-MB-231, HUVEC, and NMuMG cell lines.