Dual Emission IMage Splitter
The Cairn Optosplit image splitter is a simple device enabling a single camera to record images simultaneously at two different optical wavelengths.
The Cairn Optosplit image splitter is a simple device enabling a single camera to record images simultaneously at two different optical wavelengths. Traditionally an electronic filter changer or a second camera would be used, neither of which is ideal for all applications. The switching speed of an electronic filter changer limits the system and means that the two images are not truly synchronous. Adding a second camera to an imaging system is expensive and often unnecessarily complicated, because in many instances the region of interest does not require the full resolution of the camera. The Optosplit has been designed as a convenient, inexpensive solution to simultaneous dual wavelength imaging. It has the unique feature of a rotating mirror cradle, which gives adjustable spatial separation, to facilitate image registration. We can also offer a choice of variable field apertures to define the region of interest and prevent cross-talk between the two wavelengths.
Device drivers are included in several commercial imaging packages to assist registration and to allow realtime and off-line ratioing or fluorescence overlays. Alternatively the Optosplit can be used with simple image capture software and the processing carried out manually off-line. The simple and accessible design makes the Optosplit an excellent platform for alternative applications, such as dual polarisation imaging. Whilst optimised for coupling to a scientific microscope, the Optosplit can also be used with camera lenses or any other system of lenses which produce an image plane of suitable size.