One Shot® Mach1™ T1 Phage-Resistant Chemically Competent E. coli by Thermo Fisher Scientific Invitrogen

The Mach1™ T1 Phage-Resistant (T1R) E. coli strain is the fastest growing chemically competent strain currently available (Figure 1). Doubling time is approximately 50 minutes, compared with an excess of 74 minutes for other cloning strains. Mach1™ colonies are clearly visible within eight hours of plating the transformation mix+, enabling you to plate and pick colonies in the same day. Perform mini-preps after only 4 hours of growth from an overnight colony-saving an entire day in your experiments.

The fast-growing Mach1™-T1R strain offers the following benefits:

• Minimizes non-specific recombination in cloned DNA (recA)
• Resistance to T1 and T5 phage (tonA)
• Cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by Endonuclease I (endA1)
• Efficient transformation of unmethylated DNA from PCR amplifications (hsdR)
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• screening of recombinant clones (lacZΔM15)

Two Formats to Choose FromMach1™-T1R E. coli are available as chemically competent cells in two formats:

• The convenient One Shot® format provides single-use, 50-µl aliquots that eliminate efficiency-zapping, freeze-thaw cycles and money wasted on unused cells
• The MultiShot™ StripWell format allows rapid, high-throughput transformation in a 96-well stripwell plate that allows you to perform as few or as many transformations as you need without wasting reagents